Microcolony Size Distribution Assay Enables High-Throughput Cell Survival Quantitation
Name
1-s2.0-S2211124719300828-main.pdf
Description
Published version
Size
2.85 MB
Format
Adobe PDF
Checksum (MD5)
6e3f89721726bd1a549157f3d830986b
Author(s)
Ngo, Le P
Ge, Jing
Samson, Leona D
Engelward, Bevin P
Date Issued
February 2019
Journal
Cell Reports
Publisher
Elsevier BV
Citation
Ngo, Le P. et al. “Microcolony Size Distribution Assay Enables High-Throughput Cell Survival Quantitation.” Cell Reports 26 (2019): 1668-1678 © 2019 The Author(s)
Version
Final published version
Abstract
Cell survival is a critical and ubiquitous endpoint inbiology. The broadly accepted colony formationassay (CFA) directly measures a cell’s ability to divide;however, it takes weeks to perform and is incompat-ible with high-throughput screening (HTS) technolo-gies. Here, we describe the MicroColonyChip, whichexploits microwell array technology to create an arrayof colonies. Unlike the CFA, where visible coloniesare counted by eye, using fluorescence microscopy,microcolonies can be analyzed in days rather thanweeks. Using automated analysis of microcolonysize distributions, the MicroColonyChip achievescomparable sensitivity to the CFA (and greater sensi-tivity than the 2,3-bis-(2-methoxy-4-nitro-5-sulfo-phenyl)-2H-tetrazolium-5-carboxanilide [XTT] assay).Compared to CellTiter-Glo, the MicroColonyChip isas sensitive and also robust to artifacts caused bydifferences in initial cell seeding density. We demon-strate efficacy via studies of radiosensitivity andchemosensitivity and show that the approach isamenable to multiplexing. We conclude that theMicroColonyChip is a rapid and automated alternativefor cell survival quantitation.
Subjects
General Biochemistry, Genetics and Molecular Biology
MIT Department
Massachusetts Institute of Technology. Department of Biological Engineering
Massachusetts Institute of Technology. Department of Biology
Terms of Use
Creative Commons Attribution-NonCommercial-NoDerivs License
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1016/j.celrep.2019.01.053
