Mechanisms of nuclear segregation in a multinucleate multibudding yeast
Name
jcb_202504068.pdf
Description
Published version
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9.05 MB
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Adobe PDF
Checksum (MD5)
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Author(s)
Petrucco, Claudia A
Crocker, Alex W
Wirshing, Alison CE
Colarusso, Analeigha V
Waarts, Maya
Gladfelter, Amy S
Lew, Daniel J
Date Issued
November 3, 2025
Journal
Journal of Cell Biology
Publisher
Rockefeller University Press
Citation
Claudia A. Petrucco, Alex W. Crocker, Alison C.E. Wirshing, Analeigha V. Colarusso, Maya Waarts, Amy S. Gladfelter, Daniel J. Lew; Mechanisms of nuclear segregation in a multinucleate multibudding yeast. J Cell Biol 3 November 2025; 224 (11): e202504068.
Version
Final published version
Abstract
Budding yeasts present an especially challenging geometry for segregation of chromosomes, which must be delivered across the narrow mother–bud neck into the bud. Studies in the model yeast Saccharomyces cerevisiae have revealed an elaborate set of mechanisms that selectively orient one mitotic spindle pole toward the bud and then drive spindle elongation along the mother–bud axis, ensuring nuclear segregation between mother and bud. It is unclear how these pathways might be adapted to yield similar precision in more complex cell geometries. Here, we provide the first description of the dynamics of mitosis in a multinucleate, multibudding yeast, Aureobasidium pullulans, and identify many unexpected differences from uninucleate yeasts. Mitotic spindles do not orient along the mother–bud axis prior to anaphase, and accurate nuclear segregation often occurs after spindle disassembly. Cortical Num1–dynein forces pull highly mobile nuclei into buds, and once a nucleus enters a bud, it discourages others from entering, ensuring that most daughters inherit only one nucleus.
MIT Department
Massachusetts Institute of Technology. Department of Biology
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Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
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DOI of Published Version
https://doi.org/10.1083/jcb.202504068
