Evidence for de novo Biosynthesis of the Luminous Substrate Coelenterazine in Ctenophores
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1-s2.0-S2589004220310567-main.pdf
Description
Published version
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1.54 MB
Format
Adobe PDF
Checksum (MD5)
50fd2fc48eb91d02b44a106db2227fad
Author(s)
Bessho-Uehara, Manabu
Huang, Wentao
Patry, Wyatt L
Browne, William E
Weng, Jing-Ke
Haddock, Steven HD
Date Issued
2020
Journal
iScience
Publisher
Elsevier BV
Version
Final published version
Abstract
© 2020 The Authors Coelenterazine is a key substrate involved in marine bioluminescence which is used for light-production by at least nine phyla. Some luminous animals, such as the hydromedusa Aequorea, lack the ability to produce coelenterazine endogenously and instead depend on dietary sources. Little is known about the source organisms or the metabolic process of coelenterazine biosynthesis. Here, we present evidence that ctenophores are both producers and suppliers of coelenterazine in marine ecosystems. Using biochemical assays and mass spectrometry analyses, we detected coelenterazine from cultured ctenophores fed with a non-luminous coelenterazine-free diet. We propose that ctenophores are an emerging model organism to study coelenterazine biosynthesis and the origins of bioluminescence.
MIT Department
Whitehead Institute for Biomedical Research
Massachusetts Institute of Technology. Department of Biology
Terms of Use
Creative Commons Attribution 4.0 International license
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DOI of Published Version
https://doi.org/10.1016/j.isci.2020.101859
