Budding yeast cell cycle analysis and morphological characterization by automated image analysis
Name
755812032-MIT.pdf
Description
Full printable version
Size
7.57 MB
Format
Adobe PDF
Checksum (MD5)
206ce3ac60e6dd81c41661b41558167c
Author(s)
Perley, Elizabeth (Elizabeth Bacher)
Advisor(s)
Mark Bathe.
Alternative Title
Cellular response to DNA damage in budding yeast
Cellular response to deoxyribonucleic acid damage in budding yeast
Date Issued
2011
Publisher
Massachusetts Institute of Technology
Abstract
Budding yeast Saccharomyces cerevisiae is a standard model system for analyzing cellular response as it is related to the cell cycle. The analysis of yeast cell cycle is typically done visually or by using flow cytometry. The first of these methods is slow, while the second offers a limited amount of information about the cell's state. This thesis develops methods for automatically analyzing yeast cell morphology and yeast cell cycle using high content screening with a high-capacity automated imaging system. The images obtained using this method can also provide information about fluorescently labelled proteins, unlike flow cytometry, which can only measure overall fluorescent intensity. The information about yeast cell cycle stage and protein amount and localization can then be connected in order to develop a model of yeast cellular response to DNA damage.
Description
Thesis (M. Eng.)--Massachusetts Institute of Technology, Dept. of Electrical Engineering and Computer Science, 2011.
Cataloged from PDF version of thesis.
Includes bibliographical references (p. 70-71).
Subjects
Electrical Engineering and Computer Science.
MIT Department
Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science
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