Direct and specific chemical control of eukaryotic translation with a synthetic RNA–protein interaction
Name
Goldfless-2012-Direct and specific.pdf
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604.57 KB
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Author(s)
Goldfless, Stephen Jacob
Belmont, Brian Joshua
de Paz, Alexandra M.
Niles, Jacquin
Date Issued
January 2012
Journal
Nucleic Acids Research
Publisher
Oxford University Press (OUP)
Citation
Goldfless, S. J. et al. “Direct and Specific Chemical Control of Eukaryotic Translation with a Synthetic RNA-protein Interaction.” Nucleic Acids Research 40.9 (2012): e64–e64.
Version
Final published version
Abstract
Sequence-specific RNA–protein interactions, though commonly used in biological systems to regulate translation, are challenging to selectively modulate. Here, we demonstrate the use of a chemically-inducible RNA–protein interaction to regulate eukaryotic translation. By genetically encoding Tet Repressor protein (TetR)-binding RNA elements into the 5′-untranslated region (5′-UTR) of an mRNA, translation of a downstream coding sequence is directly controlled by TetR and tetracycline analogs. In endogenous and synthetic 5′-UTR contexts, this system efficiently regulates the expression of multiple target genes, and is sufficiently stringent to distinguish functional from non-functional RNA–TetR interactions. Using a reverse TetR variant, we illustrate the potential for expanding the regulatory properties of the system through protein engineering strategies.
MIT Department
Massachusetts Institute of Technology. Department of Biological Engineering
Terms of Use
Creative Commons Attribution Non-Commercial
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1093/nar/gks028
