Use of [superscript 18]F‑2-Fluorodeoxyglucose to Label Antibody Fragments for Immuno-Positron Emission Tomography of Pancreatic Cancer
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Ploegh_Use of 18F.pdf
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Author(s)
Keliher, Edmund J.
Dougan, Michael
Cavallari, Marco
Wojtkiewicz, Gregory R.
Jacobsen, Johanne T.
Edens, Jerre G.
Tas, Jeroen M. J.
Victora, Gabriel
Weissleder, Ralph
Rashidian, Mohammad
Date Issued
June 2015
Journal
ACS Central Science
Publisher
American Chemical Society (ACS)
Citation
Rashidian, Mohammad et al. “Use of 18 F-2-Fluorodeoxyglucose to Label Antibody Fragments for Immuno-Positron Emission Tomography of Pancreatic Cancer.” ACS Central Science 1.3 (2015): 142–147. © 2015 American Chemical Society
Version
Final published version
Abstract
We generated [superscript 18]F-labeled antibody fragments for positron emission tomography (PET) imaging using a sortase-mediated reaction to install a trans-cyclooctene-functionalized short peptide onto proteins of interest, followed by reaction with a tetrazine-labeled-[superscript 18]F-2-deoxyfluoroglucose (FDG). The method is rapid, robust, and site-specific (radiochemical yields > 25%, not decay corrected). The availability of [superscript 18]F-2-deoxyfluoroglucose avoids the need for more complicated chemistries used to generate carbon–fluorine bonds. We demonstrate the utility of the method by detecting heterotopic pancreatic tumors in mice by PET, using anti-Class II MHC single domain antibodies. We correlate macroscopic PET images with microscopic two-photon visualization of the tumor. Our approach provides easy access to [superscript 18]F-labeled antibodies and their fragments at a level of molecular specificity that complements conventional [superscript 18]F-FDG imaging.
MIT Department
Massachusetts Institute of Technology. Department of Biology
Whitehead Institute for Biomedical Research
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Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
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DOI of Published Version
https://doi.org/10.1021/acscentsci.5b00121
