Characterization of Lipid Membrane Properties for Tunable Electroporation
Author(s)
Cho, Han-Jae Jeremy
Maroo, Shalabh
Wang, Evelyn
Date Issued
March 2012
Journal
ASME 2012 Third International Conference on Micro/Nanoscale Heat and Mass Transfer
Publisher
ASME International
Citation
Cho, H. Jeremy, Shalabh C. Maroo, and Evelyn N. Wang. “Characterization of Lipid Membrane Properties for Tunable Electroporation.” ASME 2012 Third International Conference on Micro/Nanoscale Heat and Mass Transfer, 3-6 March 3, 2012, Atlanta, Georgia, ASME, 2012. © 2012 by ASME
Version
Final published version
Abstract
Lipid bilayers form nanopores on the application of an electric field. This process of electroporation can be utilized in different applications ranging from targeted drug delivery in cells to nano-gating membrane for engineering applications. However, the ease of electroporation is dependent on the surface energy of the lipid layers and thus directly related to the packing structure of the lipid molecules. 1,2- dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) lipid monolayers were deposited on a mica substrate using the Langmuir-Blodgett (LB) technique at different packing densities and analyzed using atomic force microscopy (AFM). The wetting behavior of these monolayers was investigated by contact angle measurement and molecular dynamics simulations. It was found that an equilibrium packing density of liquid-condensed (LC) phase DPPC likely exists and that water molecules can penetrate the monolayer displacing the lipid molecules. The surface tension of the monolayer in air and water was obtained along with its breakthrough force. Topics: Membranes, Electroporation
MIT Department
Massachusetts Institute of Technology. Department of Mechanical Engineering
Terms of Use
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Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1115/MNHMT2012-75321
