The future is now: single-cell genomics of bacteria and archaea
Name
Blainey_The future.pdf
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3.57 MB
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Adobe PDF
Checksum (MD5)
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Author(s)
Blainey, Paul C.
Date Issued
February 2013
Journal
FEMS Microbiology Reviews
Publisher
Wiley Blackwell
Citation
Blainey, Paul C. “The Future Is Now: Single-Cell Genomics of Bacteria and Archaea.” FEMS Microbiol Rev 37, no. 3 (May 2013): 407–427.
Version
Author's final manuscript
Abstract
Interest in the expanding catalog of uncultivated microorganisms, increasing recognition of heterogeneity among seemingly similar cells, and technological advances in whole-genome amplification and single-cell manipulation are driving considerable progress in single-cell genomics. Here, the spectrum of applications for single-cell genomics, key advances in the development of the field, and emerging methodology for single-cell genome sequencing are reviewed by example with attention to the diversity of approaches and their unique characteristics. Experimental strategies transcending specific methodologies are identified and organized as a road map for future studies in single-cell genomics of environmental microorganisms. Over the next decade, increasingly powerful tools for single-cell genome sequencing and analysis will play key roles in accessing the genomes of uncultivated organisms, determining the basis of microbial community functions, and fundamental aspects of microbial population biology.
MIT Department
Massachusetts Institute of Technology. Department of Biological Engineering
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Creative Commons Attribution-Noncommercial-Share Alike
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DOI of Published Version
https://doi.org/10.1111/1574-6976.12015
