Design, modeling, and validation of an apical flow Transwell insert for small intestinal models
Name
953868985-MIT.pdf
Description
Full printable version
Size
11.14 MB
Format
Adobe PDF
Checksum (MD5)
2c99ed5529e24c7f3ab8e180821d38e3
Author(s)
Nguyen, Transon Van
Advisor(s)
Linda G. Griffith.
Date Issued
2015
Publisher
Massachusetts Institute of Technology
Abstract
This thesis presents the design, modeling and experimental validation of a device that allows for apical flow over an in 'vitro small intestine model. The device is designed to interface with commercially available hanging Transwells. Design considerations and specifications are discussed, based on user considerations, mechanical requirements, and biological requirements. To create a more informed design, multiple phenomena are modeled and results are used to modify design characteristics and give insight into viable operating parameters, such as apical flow rate. Within the device, velocity profiles of media flow as well as oxygen transport and cellular oxygen consumption are modeled. External to the device, heat transfer from a warm incubator into room temperature tubing containing media is modeled to ensure proper media warming. Experimental validation of the flow models is done by tracking dye flow through the insert, and preliminary flow experiments appear to be in agreement with modeling results. The design, modeling, and experimental results discussed in this thesis present a promising step toward a device capable of maintaining oxygen gradients that recapitulate specific aspects of the complex, dynamic environment within the small intestine.
Description
Thesis: S.M., Massachusetts Institute of Technology, Department of Mechanical Engineering, 2015.
Cataloged from PDF version of thesis.
Includes bibliographical references (pages 79-81).
Subjects
Mechanical Engineering.
MIT Department
Massachusetts Institute of Technology. Department of Mechanical Engineering
Terms of Use
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