Identification of New Branch Points and Unconventional Introns in Saccharomyces Cerevisiae
Name
Gould-Identification of ne.pdf
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1.8 MB
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Author(s)
Gould, Genevieve Michelle
Paggi, Joseph M.
Guo, Yuchun
Phizicky, David Vincent
Zinshteyn, Boris
Wang, Eric T
Gilbert, Wendy
Gifford, David K
Burge, Christopher B
Date Issued
June 2016
Journal
RNA
Publisher
Cold Spring Harbor Laboratory Press
Citation
Gould, Genevieve M., Joseph M. Paggi, Yuchun Guo, David V. Phizicky, Boris Zinshteyn, Eric T. Wang, Wendy V. Gilbert, David K. Gifford, and Christopher B. Burge. “Identification of New Branch Points and Unconventional Introns in Saccharomyces Cerevisiae.” RNA 22, 10 (July 2016): 1522–1534 © 2016 Gould et al
Version
Final published version
Abstract
Spliced messages constitute one-fourth of expressed mRNAs in the yeast Saccharomyces cerevisiae, and most mRNAs in metazoans. Splicing requires 5′ splice site (5′SS), branch point (BP), and 3′ splice site (3′SS) elements, but the role of the BP in splicing control is poorly understood because BP identification remains difficult. We developed a high-throughput method, Branch-seq, to map BPs and 5′SSs of isolated RNA lariats. Applied to S. cerevisiae, Branch-seq detected 76% of expressed, annotated BPs and identified a comparable number of novel BPs. We performed RNA-seq to confirm associated 3′SS locations, identifying some 200 novel splice junctions, including an AT-AC intron. We show that several yeast introns use two or even three different BPs, with effects on 3′SS choice, protein coding potential, or RNA stability, and identify novel introns whose splicing changes during meiosis or in response to stress. Together, these findings show unanticipated complexity of splicing in yeast.
MIT Department
Massachusetts Institute of Technology. Computer Science and Artificial Intelligence Laboratory
Massachusetts Institute of Technology. Department of Biology
Terms of Use
Creative Commons Attribution-NonCommercial 4.0 International
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DOI of Published Version
https://doi.org/10.1261/rna.057216.116
