Green fluorescent protein as a mechanical sensor
Name
181101692-MIT.pdf
Description
Full printable version
Size
24.19 MB
Format
Adobe PDF
Checksum (MD5)
1b4430fad610cc3db3f2b399f906e10c
Author(s)
Muso, Taro M. (Taro Michael)
Advisor(s)
Paul Matsudaira.
Alternative Title
GFP as a mechanical sensor
Date Issued
2007
Publisher
Massachusetts Institute of Technology
Abstract
Inquiry into intracellular and cytoskeletal mechanics requires an intracellular mechanical sensor to verify models of sub-cellular structure dynamics. To this end, the green fluorescent protein (GFP) is considered as a mechanical sensor candidate with many desirable characteristics. Implicit solvent molecular dynamics CHARMM simulations demonstrated details inaccessible by AFM and OT methods, such as the linkage dependency of fluorophore environment changes and the energy exchanges between protein components during protein unfolding. Theoretical considerations and in vitro experiments explored the parameters important to GFP conjugation by N-hydroxysuccinimide (NHS) ester chemistry, and the complexities associated with a polymer approach to a controlled distribution of force across fluorescent proteins in a polyacrylamide (PAM) gel.
Description
Thesis (S.M.)--Harvard-MIT Division of Health Sciences and Technology, 2007.
Includes bibliographical references (p. 133-141).
Subjects
Harvard University--MIT Division of Health Sciences and Technology.
MIT Department
Harvard University--MIT Division of Health Sciences and Technology
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