Confinement to Organelle-Associated Inclusion Structures Mediates Asymmetric Inheritance of Aggregated Protein in Budding Yeast
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England_Confinement to organelle.pdf
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Author(s)
Spokoini, Rachel
Moldavski, Ofer
Nahmias, Yaakov
England, Jeremy L.
Schuldiner, Maya
Kaganovich, Daniel
Date Issued
September 2012
Journal
Cell Reports
Publisher
Elsevier
Citation
Spokoini, Rachel et al. “Confinement to Organelle-Associated Inclusion Structures Mediates Asymmetric Inheritance of Aggregated Protein in Budding Yeast.” Cell Reports 2.4 (2012): 738–747.
Version
Final published version
Abstract
The division of the S. cerevisiae budding yeast, which produces one mother cell and one daughter cell, is asymmetric with respect to aging. Remarkably, the asymmetry of yeast aging coincides with asymmetric inheritance of damaged and aggregated proteins by the mother cell. Here, we show that misfolded proteins are retained in the mother cell by being sequestered in juxtanuclear quality control compartment (JUNQ) and insoluble protein deposit (IPOD) inclusions, which are attached to organelles. Upon exposure to stress, misfolded proteins accumulate in stress foci that must be disaggregated by Hsp104 in order to be degraded or processed to JUNQ and IPOD. Cells that fail to deliver aggregates to an inclusion pass on aggregates to subsequent generations.
MIT Department
Massachusetts Institute of Technology. Department of Physics
Terms of Use
Creative Commons Attribution-Noncommercial-No Derivative Works
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DOI of Published Version
https://doi.org/10.1016/j.celrep.2012.08.024
