RNA Polymerase II cluster dynamics predict mRNA output in living cells
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RNA polymerase II.pdf
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3.93 MB
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Author(s)
English, Brian P
Grimm, Jonathan B
Lavis, Luke D
Lionnet, Timothée
Cho, Won-ki
Jayanth, Namrata
Andrews, James Owen
Conway, William E.
Spille, Jan Hendrik
Cisse, Ibrahim I
Date Issued
May 2016
Journal
eLife
Publisher
eLife Sciences Publications, Ltd
Citation
Cho, Won-Ki; Jayanth, Namrata; English, Brian P; Inoue, Takuma; Andrews, J Owen; Conway, William; Grimm, Jonathan B et al. “RNA Polymerase II Cluster Dynamics Predict mRNA Output in Living Cells.” eLife 5 (May 3, 2016). © Cho et al.
Version
Final published version
Abstract
Protein clustering is a hallmark of genome regulation in mammalian cells. However, the dynamic molecular processes involved make it difficult to correlate clustering with functional consequences in vivo. We developed a live-cell super-resolution approach to uncover the correlation between mRNA synthesis and the dynamics of RNA Polymerase II (Pol II) clusters at a gene locus. For endogenous β-actin genes in mouse embryonic fibroblasts, we observe that short-lived (~8 s) Pol II clusters correlate with basal mRNA output. During serum stimulation, a stereotyped increase in Pol II cluster lifetime correlates with a proportionate increase in the number of mRNAs synthesized. Our findings suggest that transient clustering of Pol II may constitute a pre-transcriptional regulatory event that predictably modulates nascent mRNA output.
MIT Department
Massachusetts Institute of Technology. Department of Physics
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Creative Commons Attribution 4.0 International License
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DOI of Published Version
https://doi.org/10.7554/eLife.13617
