Diversity and evolution of class 2 CRISPR–Cas systems
Name
nihms945745.pdf
Description
Accepted version
Size
1.97 MB
Format
Adobe PDF
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Author(s)
Shmakov, Sergey
Smargon, Aaron Andrew
Scott, David (David Arthur)
Cox, David D.
Pyzocha, Neena
Yan, Winston Xia
Abudayyeh, Omar O.
Gootenberg, Jonathan S
Makarova, Kira S.
Wolf, Yuri I.
Date Issued
January 2017
Journal
Nature Reviews Microbiology
Publisher
Springer Science and Business Media LLC
Citation
Shmakov, Sergey et al. "Diversity and evolution of class 2 CRISPR–Cas systems." Nature Reviews Microbiology 15, 3 (March 2017): 169–182
Version
Author's final manuscript
Abstract
Class 2 CRISPR-Cas systems are characterized by effector modules that consist of a single multidomain protein, such as Cas9 or Cpf1. We designed a computational pipeline for the discovery of novel class 2 variants and used it to identify six new CRISPR-Cas subtypes. The diverse properties of these new systems provide potential for the development of versatile tools for genome editing and regulation. In this Analysis article, we present a comprehensive census of class 2 types and class 2 subtypes in complete and draft bacterial and archaeal genomes, outline evolutionary scenarios for the independent origin of different class 2 CRISPR-Cas systems from mobile genetic elements, and propose an amended classification and nomenclature of CRISPR-Cas. Keywords:
Bacterial evolution; Bacterial genetics; CRISPR-Cas systems
MIT Department
Broad Institute of MIT and Harvard
Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science
Massachusetts Institute of Technology. Department of Biology
Harvard University--MIT Division of Health Sciences and Technology
McGovern Institute for Brain Research at MIT
Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences
Massachusetts Institute of Technology. Department of Biological Engineering
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Creative Commons Attribution-Noncommercial-Share Alike
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DOI of Published Version
https://doi.org/10.1038/nrmicro.2016.184
