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Trade-offs in Robustness to Perturbations of Bacterial Population Controllers *
Name
McBride_ACC2020_final.pdf
Description
Accepted version
Size
355.29 KB
Format
Adobe PDF
Checksum (MD5)
e8b34d9e8659c3704ec1d31ecc76533e
Author(s)
McBride, Cameron
Del Vecchio, Domitilla
Date Issued
2020
Journal
Proceedings of the American Control Conference
Publisher
Institute of Electrical and Electronics Engineers (IEEE)
Citation
McBride, Cameron and Del Vecchio, Domitilla. 2020. "Trade-offs in Robustness to Perturbations of Bacterial Population Controllers *." Proceedings of the American Control Conference, 2020-July.
Version
Author's final manuscript
Abstract
Synthetic biology applications have the potential to have lasting impact; however, there is considerable difficulty in scaling up engineered genetic circuits. One of the current hurdles is resource sharing, where different circuit components become implicitly coupled through the host cell's pool of resources, which may destroy circuit function. One potential solution around this problem is to distribute genetic circuit components across multiple cell strains and control the cell population size using a population controller. In these situations, perturbations in the availability of cellular resources, such as due to resource sharing, will affect the performance of the population controller. In this work, we model a genetic population controller implemented by a genetic circuit while considering perturbations in the availability of cellular resources. We analyze how these intracellular perturbations and extracellular disturbances to cell growth affect cell population size. We find that it is not possible to tune the population controller's gain such that the population density is robust to both extracellular disturbances and perturbations to the pool of available resources.
Terms of Use
Creative Commons Attribution-Noncommercial-Share Alike
Persistent DSpace Link
DOI of Published Version
10.23919/ACC45564.2020.9148039
