Light and Hydrogen Peroxide Inhibit C. elegans Feeding through Gustatory Receptor Orthologs and Pharyngeal Neurons
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Horvitz_Light and hydrogen.pdf
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Author(s)
Bhatla, Nikhil
Horvitz, Howard Robert
Date Issued
January 2015
Journal
Neuron
Publisher
Elsevier/Cell Press
Citation
Bhatla, Nikhil, and H. Robert Horvitz. “Light and Hydrogen Peroxide Inhibit C. Elegans Feeding through Gustatory Receptor Orthologs and Pharyngeal Neurons.” Neuron 85.4 (2015): 804–818.
Version
Author's final manuscript
Abstract
While gustatory sensing of the five primary flavors (sweet, salty, sour, bitter, and savory) has been extensively studied, pathways that detect non-canonical taste stimuli remain relatively unexplored. In particular, while reactive oxygen species cause generalized damage to biological systems, no gustatory mechanism to prevent ingestion of such material has been identified in any organism. We observed that light inhibits C. elegans feeding and used light as a tool to uncover molecular and neural mechanisms for gustation. Light can generate hydrogen peroxide, and we discovered that hydrogen peroxide similarly inhibits feeding. The gustatory receptor family members LITE-1 and GUR-3 are required for the inhibition of feeding by light and hydrogen peroxide. The I2 pharyngeal neurons increase calcium in response to light and hydrogen peroxide, and these
responses require GUR-3 and a conserved antioxidant enzyme peroxiredoxin PRDX-2. Our results demonstrate a gustatory mechanism that mediates the detection and blocks ingestion of a noncanonical
taste stimulus, hydrogen peroxide.
MIT Department
Massachusetts Institute of Technology. Department of Biology
Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences
McGovern Institute for Brain Research at MIT
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Creative Commons Attribution-NonCommercial-NoDerivs License
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DOI of Published Version
https://doi.org/10.1016/j.neuron.2014.12.061
