An engineered live biotherapeutic for the prevention of antibiotic-induced dysbiosis
Name
eLBP_AntibioticDysbiosis_Manuscript_PR.pdf
Description
Accepted version
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1.25 MB
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Adobe PDF
Checksum (MD5)
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Author(s)
Cubillos-Ruiz, Andrés
Alcantar, Miguel A
Donghia, Nina M
Cárdenas, Pablo
Avila-Pacheco, Julian
Collins, James J
Date Issued
2022
Journal
Nature Biomedical Engineering
Publisher
Springer Science and Business Media LLC
Citation
Cubillos-Ruiz, Andrés, Alcantar, Miguel A, Donghia, Nina M, Cárdenas, Pablo, Avila-Pacheco, Julian et al. 2022. "An engineered live biotherapeutic for the prevention of antibiotic-induced dysbiosis." Nature Biomedical Engineering, 6 (7).
Version
Author's final manuscript
Abstract
Antibiotic-induced alterations in the gut microbiota are implicated in many metabolic and inflammatory diseases, increase the risk of secondary infections and contribute to the emergence of antimicrobial resistance. Here we report the design and in vivo performance of an engineered strain of Lactococcus lactis that altruistically degrades the widely used broad-spectrum antibiotics β-lactams (which disrupt commensal bacteria in the gut) through the secretion and extracellular assembly of a heterodimeric β-lactamase. The engineered β-lactamase-expression system does not confer β-lactam resistance to the producer cell, and is encoded via a genetically unlinked two-gene biosynthesis strategy that is not susceptible to dissemination by horizontal gene transfer. In a mouse model of parenteral ampicillin treatment, oral supplementation with the engineered live biotherapeutic minimized gut dysbiosis without affecting the ampicillin concentration in serum, precluded the enrichment of antimicrobial resistance genes in the gut microbiome and prevented the loss of colonization resistance against Clostridioides difficile. Engineered live biotherapeutics that safely degrade antibiotics in the gut may represent a suitable strategy for the prevention of dysbiosis and its associated pathologies.
MIT Department
Massachusetts Institute of Technology. Department of Biological Engineering
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Creative Commons Attribution-Noncommercial-Share Alike
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DOI of Published Version
https://doi.org/10.1038/S41551-022-00871-9
