Stable Force Balance between Epithelial Cells Arises from F-Actin Turnover
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Stable force.pdf
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Author(s)
Perkins, Lizabeth A.
Perrimon, Norbert
Jodoin, Jeanne
Coravos, Jonathan Stuck
Chanet, Soline
Kingston, Elena
Martin, Adam C
Vasquez, Claudia G
Tworoger, Michael B.
Date Issued
December 2015
Journal
Developmental Cell
Publisher
Elsevier
Citation
Jodoin, Jeanne N., Jonathan S. Coravos, Soline Chanet, Claudia G. Vasquez, Michael Tworoger, Elena R. Kingston, Lizabeth A. Perkins, Norbert Perrimon, and Adam C. Martin. “Stable Force Balance Between Epithelial Cells Arises from F-Actin Turnover.” Developmental Cell 35, no. 6 (December 2015): 685–697.
Version
Author's final manuscript
Abstract
The propagation of force in epithelial tissues requires that the contractile cytoskeletal machinery be stably connected between cells through E-cadherin-containing adherens junctions. In many epithelial tissues, the cells’ contractile network is positioned at a distance from the junction. However, the mechanism or mechanisms that connect the contractile networks to the adherens junctions, and thus mechanically connect neighboring cells, are poorly understood. Here, we identified the role for F-actin turnover in regulating the contractile cytoskeletal network’s attachment to adherens junctions. Perturbing F-actin turnover via gene depletion or acute drug treatments that slow F-actin turnover destabilized the attachment between the contractile actomyosin network and adherens junctions. Our work identifies a critical role for F-actin turnover in connecting actomyosin to intercellular junctions, defining a dynamic process required for the stability of force balance across intercellular contacts in tissues.
MIT Department
Massachusetts Institute of Technology. Department of Biology
Terms of Use
Creative Commons Attribution-NonCommercial-NoDerivs License
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DOI of Published Version
https://doi.org/10.1016/j.devcel.2015.11.018
