Digital micromirror device-based common-path quantitative phase imaging
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Author(s)
Zheng, Cheng
Zhou, Renjie
Kuang, Cuifang
Zhao, Guangyuan
Yaqoob, Zahid
So, Peter T. C.
Date Issued
March 2017
Journal
Optics Letters
Publisher
The Optical Society
Citation
Zheng, Cheng, Renjie Zhou, Cuifang Kuang, Guangyuan Zhao, Zahid Yaqoob, and Peter T. C. So. “Digital Micromirror Device-Based Common-Path Quantitative Phase Imaging.” Optics Letters 42, no. 7 (March 31, 2017): 1448. © 2017 Optical Society of America.
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Author's final manuscript
Abstract
We propose a novel common-path quantitative phase imaging (QPI) method based on a digital micromirror device (DMD). The DMD is placed in a plane conjugate to the objective back-aperture plane for the purpose of generating two plane waves thatilluminate the sample.Apinhole isused in the detection arm to filter one of the beams after sample to create a reference beam. Additionally, a transmission-type liquid crystal device, placed at the objective back-aperture plane, eliminates the specular reflection noise arising from all the "off" state DMD micromirrors, which is common in all DMD-based illuminations. We have demonstrated high sensitivity QPI, which has a measured spatial and temporal noise of 4.92 nm and 2.16 nm, respectively. Experiments with calibrated polystyrene beads illustrate the desired phase measurement accuracy. In addition, we have measured the dynamic height maps of red blood cell membrane fluctuations, showing the efficacy of the proposed system for live cell imaging. Most importantly, the DMD grants the system convenience in varying the interference fringe period on the camera to easily satisfy the pixel sampling conditions. This feature also alleviates the pinhole alignment complexity. We envision that the proposed DMD-based common-path QPI system will allow for system miniaturization and automation for a broader adaption.
MIT Department
Massachusetts Institute of Technology. Department of Biological Engineering
Massachusetts Institute of Technology. Department of Chemistry
Massachusetts Institute of Technology. Department of Mechanical Engineering
Massachusetts Institute of Technology. Laser Biomedical Research Center
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DOI of Published Version
https://doi.org/10.1364/OL.42.001448
