Nanolayered siRNA Dressing for Sustained Localized Knockdown
Name
Hammond_Nanolayered siRNA.pdf
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2.96 MB
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Author(s)
Wang, Mary
Hammond, Paula T
Castleberry, Steven A
Date Issued
May 2013
Journal
ACS Nano
Publisher
American Chemical Society (ACS)
Citation
Castleberry, Steven, Mary Wang, and Paula T. Hammond. “Nanolayered siRNA Dressing for Sustained Localized Knockdown.” ACS Nano 7, no. 6 (June 25, 2013): 5251–5261.
Version
Author's final manuscript
Abstract
The success of RNA interference (RNAi) in medicine relies on the development of technology capable of successfully delivering it to tissues of interest. Significant research has focused on the difficult task of systemic delivery of RNAi; however its local delivery could be a more easily realized approach. Localized delivery is of particular interest for many medical applications, including the treatment of localized diseases, the modulation of cellular response to implants or tissue engineering constructs, and the management of wound healing and regenerative medicine. In this work we present an ultrathin electrostatically assembled coating for localized and sustained delivery of short interfering RNA (siRNA). This film was applied to a commercially available woven nylon dressing commonly used for surgical applications and was demonstrated to sustain significant knockdown of protein expression in multiple cell types for more than one week in vitro. Significantly, this coating can be easily applied to a medically relevant device and requires no externally delivered transfection agents for effective delivery of siRNA. These results present promising opportunities for the localized administration of RNAi.
MIT Department
Harvard University--MIT Division of Health Sciences and Technology
Massachusetts Institute of Technology. Department of Chemical Engineering
Koch Institute for Integrative Cancer Research at MIT
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Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
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DOI of Published Version
https://doi.org/10.1021/nn401011n
