Papain gel containing methylene blue for simultaneous caries removal and antimicrobial photoinactivation against Streptococcus mutans biofilms
Name
Papain gel.pdf
Size
1.71 MB
Format
Adobe PDF
Checksum (MD5)
7510f20c68004ab37d7dba5550075cfd
Author(s)
Silva, Zenildo Santos
Huang, Ying-Ying
de Freitas, Lucas Freitas
França, Cristiane Miranda
Botta, Sergio Brossi
Ana, Patrícia Aparecida
Mesquita-Ferrari, Raquel Agnelli
Santos Fernandes, Kristianne Porta
Deana, Alessandro
Lima Leal, Cintia Raquel
Date Issued
August 2016
Journal
Scientific Reports
Publisher
Nature Publishing Group
Citation
Silva, Zenildo Santos et al. “Papain Gel Containing Methylene Blue for Simultaneous Caries Removal and Antimicrobial Photoinactivation against Streptococcus Mutans Biofilms.” Scientific Reports 6.1 (2016): n. pag.
Version
Final published version
Abstract
This study intended to evaluate the effects of a papain-gel with a red-light absorbing pigment (methylene blue – MB) to mediate photodynamic therapy (PDT) against Streptococcus mutans biofilms. The PapaMBlue was compared with free MB to generate reactive oxygen species using fluorescence probes (SOSG and HPF). PDT (660-nm light) was carried out against S. mutans biofilms grown on either plastic dishes or on collagen membrane and assayed by CFU, live-dead staining using confocal microscopy, transmission electron microscopy and H&E staining for collagen films. Cytotoxicity and subcellular localization was studied in human fibroblasts. Sponges of bioabsorbable type I collagen membrane were exposed to papain based gel, irradiated with laser and analyzed about their integrity by ATR-FTIR. The PapaMBlue produced higher amounts of singlet oxygen and hydroxyl radicals than free MB, possibly due to better disaggregation of the dye in solution. The PapaMBlue antimicrobial effects on biofilms proved to be capable of reducing the S. mutans. Both MTT and PrestoBlue assays showed higher cell viability and metabolism scores in fibroblasts treated with PapaMBlue and MB, possibly due to stimulation of mitochondrial activity and that collagen triple helix is unaffected. The PapaMBlue is equally effective as MB in destroying S. mutans biofilms growing on plastic or collagen without affecting fibroblasts.
MIT Department
Harvard University--MIT Division of Health Sciences and Technology
Terms of Use
Creative Commons Attribution 4.0 International License
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DOI of Published Version
https://doi.org/10.1038/srep33270
