Structural changes in a marine podovirus associated with release of its genome into Prochlorococcus
Name
CHIU Nature SMB 2010 text.pdf
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1.6 MB
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Author(s)
Liu, Xiangan
Zhang, Qinfen
Murata, Kazuyoshi
Baker, Matthew L.
Sullivan, Matthew B.
Fu, Caroline
Dougherty, Matthew
Schmid, Michael F.
Osburne, Marcia
Chisholm, Sallie (Penny)
Date Issued
June 2010
Journal
Nature Structural and Molecular Biology
Publisher
Nature Publishing Group
Citation
Liu, Xiangan et al. “Structural changes in a marine podovirus associated with release of its genome into Prochlorococcus.” Nat Struct Mol Biol 17.7 (2010): 830-836.
Version
Author's final manuscript
Abstract
Podovirus P-SSP7 infects Prochlorococcus marinus, the most abundant oceanic
photosynthetic microorganism. Single particle cryo-electron microscopy (cryo-EM) yields
icosahedral and asymmetrical structures of infectious P-SSP7 with 4.6 Å and 9 Å resolution,
respectively. The asymmetric reconstruction reveals how symmetry mismatches are
accommodated among 5 of the gene products at the portal vertex. Reconstructions of
infectious and empty particles show a conformational change of the “valve” density in the
nozzle, an orientation difference in the tail fibers, a disordering of the C-terminus of the
portal protein, and disappearance of the core proteins. In addition, cryo-electron tomography
(cryo-ET) of P-SSP7 infecting Prochlorococcus demonstrated the same tail fiber conformation
as in empty particles. Our observations suggest a mechanism whereby, upon binding to the
host cell, the tail fibers induce a cascade of structural alterations of the portal vertex complex that triggers DNA release.
MIT Department
Massachusetts Institute of Technology. Department of Civil and Environmental Engineering
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DOI of Published Version
https://doi.org/10.1038/nsmb.1823
