Rapid Total Synthesis of DARPin pE59 and RNase B. a
Name
Pentelute_Rapid total.pdf
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2.95 MB
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Adobe PDF
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Author(s)
Vinogradov, Alexander A.
Pentelute, Bradley L.
Mong, Surin Khai
Simon, Mark
Alternative Title
Rapid Total Synthesis of DARPin pE59 and Barnase
Date Issued
March 2014
Journal
ChemBioChem
Publisher
Wiley Blackwell
Citation
Mong, Surin K., Alexander A. Vinogradov, Mark D. Simon, and Bradley L. Pentelute. “Rapid Total Synthesis of DARPin pE59 and Barnase.” ChemBioChem 15, no. 5 (March 11, 2014): 721–733.
Version
Author's final manuscript
Abstract
We report the convergent total synthesis of two proteins: DARPin pE59 and Bacillus amyloliquefaciens RNase (Barnase). Leveraging our recently developed fast-flow peptide-synthesis platform, we rapidly explored numerous conditions for the assembly of long polypeptides, and were able to mitigate common side reactions, including deletion and aspartimide products. We report general strategies for improving the synthetic quality of difficult peptide sequences with our system. High-quality protein fragments produced under optimal synthetic conditions were subjected to convergent native chemical ligation, which afforded native full-length proteins after a final desulfurization step. Both DARPin and Barnase were folded and found to be as active as their recombinant analogues.
MIT Department
Massachusetts Institute of Technology. Department of Chemistry
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Creative Commons Attribution-Noncommercial-Share Alike
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DOI of Published Version
https://doi.org/10.1002/cbic.201300797
