Optogenetic Inactivation Modifies Monkey Visuomotor Behavior
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Boyden_Optogenetic Inactivation.pdf
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Author(s)
Cavanaugh, James
Monosov, Ilya E.
McAlonan, Kerry
Berman, Rebecca
Smith, Mitchell K.
Cao, Vania
Wang, Kuan H.
Boyden, Edward Stuart
Wurtz, Robert H.
Date Issued
December 2012
Journal
Neuron
Publisher
Elsevier
Citation
Cavanaugh, James, Ilya E. Monosov, Kerry McAlonan, Rebecca Berman, Mitchell K. Smith, Vania Cao, Kuan H. Wang, Edward S. Boyden, and Robert H. Wurtz. “Optogenetic Inactivation Modifies Monkey Visuomotor Behavior.” Neuron 76, no. 5 (December 2012): 901–907.
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Author's final manuscript
Abstract
A critical technique for understanding how neuronal activity contributes to behavior is determining whether perturbing it changes behavior. The advent of optogenetic techniques allows the immediately reversible alteration of neuronal activity in contrast to chemical approaches lasting minutes to hours. Modification of behavior using optogenetics has had substantial success in rodents but has not been as successful in monkeys. Here, we show how optogenetic inactivation of superior colliculus neurons in awake monkeys leads to clear and repeatable behavioral deficits in the metrics of saccadic eye movements. We used our observations to evaluate principles governing the use of optogenetic techniques in the study of the neuronal bases of behavior in monkeys, particularly how experimental design must address relevant parameters, such as the application of light to subcortical structures, the spread of viral injections, and the extent of neuronal inactivation with light.
MIT Department
Massachusetts Institute of Technology. Department of Biological Engineering
Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences
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Creative Commons Attribution-NonCommercial-NoDerivs License
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DOI of Published Version
https://doi.org/10.1016/j.neuron.2012.10.016
