Skip to main content
Log in
(current)
MIT Open Scholarship
DSpace@MIT
Search
Home
MIT Open Access Articles
MIT Open Access Articles
Continuous bioactivity-dependent evolution of an antibiotic biosynthetic pathway
This is not the latest version of this item. The latest version can be found
here
.
Continuous bioactivity-dependent evolution of an antibiotic biosynthetic pathway
Download
Name
s41467-020-18018-2.pdf
Description
Published version
Show more
Size
685.3 KB
Format
Adobe PDF
Checksum
(MD5)
5b92268b5fc2b7315be038084ddc84f8
Show more
sword-2021-08-25T18:11:48.original.xml (130 B)
Original SWORD entry document
Show more
Author(s)
Johnston, Chad W
•
Badran, Ahmed H
•
Collins, James J
Date Issued
2020
Journal
Nature Communications
Publisher
Springer Science and Business Media LLC
Version
Final published version
Abstract
© 2020, The Author(s). Antibiotic biosynthetic gene clusters (BGCs) produce bioactive metabolites that impart a fitness advantage to their producer, providing a mechanism for natural selection. This selection drives antibiotic evolution and adapts BGCs for expression in different organisms, potentially providing clues to improve heterologous expression of antibiotics. Here, we use phage-assisted continuous evolution (PACE) to achieve bioactivity-dependent adaptation of the BGC for the antibiotic bicyclomycin (BCM), facilitating improved production in a heterologous host. This proof-of-principle study demonstrates that features of natural bioactivity-dependent evolution can be engineered to access unforeseen routes of improving metabolic pathways and product yields.
Show more
Terms of Use
Creative Commons Attribution 4.0 International license
https://creativecommons.org/licenses/by/4.0/
Persistent DSpace Link
https://hdl.handle.net/1721.1/133496
DOI of Published Version
10.1038/S41467-020-18018-2
