Technical Challenges in Using Human Induced Pluripotent Stem Cells to Model Disease
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Saha-2009-Technical Challenges.pdf
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717.77 KB
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Author(s)
Saha, Krishanu
Jaenisch, Rudolf
Date Issued
December 2009
Journal
Cell Stem Cell
Publisher
Elsevier
Citation
Saha, Krishanu, and Rudolf Jaenisch. “Technical Challenges in Using Human Induced Pluripotent Stem Cells to Model Disease.” Cell Stem Cell 5, no. 6 (December 2009): 584–595. © 2009 Elsevier Inc.
Version
Final published version
Abstract
Reprogramming of human somatic cells uses readily accessible tissue, such as skin or blood, to generate embryonic-like induced pluripotent stem cells (iPSCs). This procedure has been applied to somatic cells from patients who are classified into a disease group, thus creating “disease-specific” iPSCs. Here, we examine the challenges and assumptions in creating a disease model from a single cell of the patient. Both the kinetics of disease onset and progression as well as the spatial localization of disease in the patient's body are challenges to disease modeling. New tools in genetic modification, reprogramming, biomaterials, and animal models can be used for addressing these challenges.
MIT Department
Massachusetts Institute of Technology. Department of Biology
Whitehead Institute for Biomedical Research
Terms of Use
Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
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DOI of Published Version
https://doi.org/10.1016/j.stem.2009.11.009
