Circular RNA migration in agarose gel electrophoresis
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1-s2.0-S1097276522002179-main.pdf
Description
Published version
Size
2.71 MB
Format
Adobe PDF
Checksum (MD5)
9315fbe8f4b19b6b9761aef08a51817c
Author(s)
Abe, Brian T
Wesselhoeft, R Alexander
Chen, Robert
Anderson, Daniel G
Chang, Howard Y
Date Issued
May 2022
Journal
Molecular Cell
Publisher
Elsevier BV
Citation
Abe, Brian T, Wesselhoeft, R Alexander, Chen, Robert, Anderson, Daniel G and Chang, Howard Y. 2022. "Circular RNA migration in agarose gel electrophoresis." Molecular Cell, 82 (9).
Version
Final published version
Abstract
Circular RNAs are garnering increasing interest as potential regulatory RNAs and a format for gene expression. The characterization of circular RNA using analytical techniques commonly employed in the literature, such as gel electrophoresis, can, under differing conditions, yield different results when attempting to distinguish circular RNA from linear RNA of similar molecular weights. Here, we describe circular RNA migration in different conditions, analyzed by gel electrophoresis and high-performance liquid chromatography (HPLC). We characterize key parameters that affect the migration pattern of circular RNA in gel electrophoresis systems, which include gel type, electrophoresis time, sample buffer composition, and voltage. Finally, we demonstrate the utility of orthogonal analytical tests for circular RNA that take advantage of its covalently closed structure to further distinguish circular RNA from linear RNA following in vitro synthesis.
MIT Department
Massachusetts Institute of Technology. Department of Chemical Engineering
Massachusetts Institute of Technology. Institute for Medical Engineering & Science
Koch Institute for Integrative Cancer Research at MIT
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Creative Commons Attribution
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DOI of Published Version
https://doi.org/10.1016/j.molcel.2022.03.008
