Microtubules promote intercellular contractile force transmission during tissue folding
Name
jcb_201902011.pdf
Description
Published version
Size
4.5 MB
Format
Adobe PDF
Checksum (MD5)
f86370d96c355921dde25c5def005987
Author(s)
Ko, Clint S.
Tserunyan, Vardges
Martin, Adam C.
Date Issued
April 2019
Journal
Journal of cell biology
Publisher
Rockefeller University Press
Citation
Ko, Clint S., Vardges Tserunyan and Adam C. Martin. “Microtubules promote intercellular contractile force transmission during tissue folding.” Journal of cell biology 218 (2019): 2726-2742 © 2019 The Author(s)
Version
Final published version
Abstract
During development, forces transmitted between cells are critical for sculpting epithelial tissues. Actomyosin contractility in the middle of the cell apex (medioapical) can change cell shape (e.g., apical constriction) but can also result in force transmission between cells via attachments to adherens junctions. How actomyosin networks maintain attachments to adherens junctions under tension is poorly understood. Here, we discovered that microtubules promote actomyosin intercellular attachments in epithelia during Drosophila melanogaster mesoderm invagination. First, we used live imaging to show a novel arrangement of the microtubule cytoskeleton during apical constriction: medioapical Patronin (CAMSAP) foci formed by actomyosin contraction organized an apical noncentrosomal microtubule network. Microtubules were required for mesoderm invagination but were not necessary for initiating apical contractility or adherens junction assembly. Instead, microtubules promoted connections between medioapical actomyosin and adherens junctions. These results delineate a role for coordination between actin and microtubule cytoskeletal systems in intercellular force transmission during tissue morphogenesis.
MIT Department
Massachusetts Institute of Technology. Department of Biology
Terms of Use
Creative Commons Attribution-Noncommercial-Share Alike
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DOI of Published Version
https://doi.org/10.1083/JCB.201902011
