Solid-state NMR investigations of cellulose structure and interactions with matrix polysaccharides in plant primary cell walls
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JEXBOT-2015-152876v2-Hong.pdf
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642.37 KB
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Checksum (MD5)
27541aba234414d9f4f830cdf9689b09
Author(s)
Wang, Tuo
Hong, Mei
Date Issued
September 2015
Journal
Journal of Experimental Botany
Publisher
Oxford University Press
Citation
Wang, Tuo, and Mei Hong. “Solid-State NMR Investigations of Cellulose Structure and Interactions with Matrix Polysaccharides in Plant Primary Cell Walls.” Journal of Experimental Botany 67, no. 2 (September 9, 2015): 503–514.
Version
Author's final manuscript
Abstract
Until recently, the 3D architecture of plant cell walls was poorly understood due to the lack of high-resolution techniques for characterizing the molecular structure, dynamics, and intermolecular interactions of the wall polysaccharides in these insoluble biomolecular mixtures. We introduced multidimensional solid-state NMR (SSNMR) spectroscopy, coupled with [superscript 13]C labelling of whole plants, to determine the spatial arrangements of macromolecules in near-native plant cell walls. Here we review key evidence from 2D and 3D correlation NMR spectra that show relatively few cellulose–hemicellulose cross peaks but many cellulose–pectin cross peaks, indicating that cellulose microfibrils are not extensively coated by hemicellulose and all three major polysaccharides exist in a single network rather than two separate networks as previously proposed. The number of glucan chains in the primary-wall cellulose microfibrils has been under active debate recently. We show detailed analysis of quantitative [superscript 13]C SSNMR spectra of cellulose in various wild-type (WT) and mutant Arabidopsis and Brachypodium primary cell walls, which consistently indicate that primary-wall cellulose microfibrils contain at least 24 glucan chains.
MIT Department
Massachusetts Institute of Technology. Department of Chemistry
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Creative Commons Attribution-Noncommercial-Share Alike
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DOI of Published Version
https://doi.org/10.1093/jxb/erv416
