Living Bacterial Sacrificial Porogens to Engineer Decellularized Porous Scaffolds
Name
Xu-2011-Living Bacterial Sac.pdf
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1.16 MB
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Author(s)
Xu, Feng
Sridharan, BanuPriya
Durmus, Naside Gozde
Wang, ShuQi
Yavuz, Ahmet Sinan
Gurkan, Umut Atakan
Demirci, Utkan
Date Issued
April 2011
Journal
PLoS ONE
Publisher
Public Library of Science
Citation
Xu, Feng et al. “Living Bacterial Sacrificial Porogens to Engineer Decellularized Porous Scaffolds.” Ed. Che John Connon. PLoS ONE 6.4 (2011) : e19344.
Version
Final published version
Abstract
Decellularization and cellularization of organs have emerged as disruptive methods in tissue engineering and regenerative medicine. Porous hydrogel scaffolds have widespread applications in tissue engineering, regenerative medicine and drug discovery as viable tissue mimics. However, the existing hydrogel fabrication techniques suffer from limited control over pore interconnectivity, density and size, which leads to inefficient nutrient and oxygen transport to cells embedded in the scaffolds. Here, we demonstrated an innovative approach to develop a new platform for tissue engineered constructs using live bacteria as sacrificial porogens. E.coli were patterned and cultured in an interconnected three-dimensional (3D) hydrogel network. The growing bacteria created interconnected micropores and microchannels. Then, the scafold was decellularized, and bacteria were eliminated from the scaffold through lysing and washing steps. This 3D porous network method combined with bioprinting has the potential to be broadly applicable and compatible with tissue specific applications allowing seeding of stem cells and other cell types.
MIT Department
Harvard University--MIT Division of Health Sciences and Technology
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Creative Commons Attribution
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DOI of Published Version
https://doi.org/10.1371/journal.pone.0019344
