Multiplexed rapid antigen tests developed using multicolored nanoparticles and cross-reactive antibody pairs: Implications for pandemic preparedness
Name
1-s2.0-S1748013222002973-main.pdf
Description
Published version
Size
5 MB
Format
Adobe PDF
Checksum (MD5)
109c8bdba25aae108de65f63610b2cde
Author(s)
de Puig, Helena
Bosch, Irene
Salcedo, Nol
Collins, James J
Hamad-Schifferli, Kimberly
Gehrke, Lee
Date Issued
December 2022
Journal
Nano Today
Publisher
Elsevier BV
Citation
de Puig, Helena, Bosch, Irene, Salcedo, Nol, Collins, James J, Hamad-Schifferli, Kimberly et al. 2022. "Multiplexed rapid antigen tests developed using multicolored nanoparticles and cross-reactive antibody pairs: Implications for pandemic preparedness." Nano Today, 47.
Version
Final published version
Abstract
Global public health infrastructure is unprepared for emerging pathogen epidemics, in part because diagnostic tests are not developed in advance. The recent Zika, Ebola, and SARS-CoV-2 virus epidemics are cases in point. We demonstrate here that multicolored gold nanoparticles, when coupled to cross-reactive monoclonal antibody pairs generated from a single immunization regimen, can be used to create multiple diagnostics that specifically detect and distinguish related viruses. The multiplex approach for specific detection centers on immunochromatography with pairs of antibody-conjugated red and blue gold nanoparticles, coupled with clustering algorithms to detect and distinguish related pathogens. Cross-reactive antibodies were used to develop rapid tests for i) Dengue virus serotypes 1-4, ii) Zika virus, iii) Ebola and Marburg viruses, and iv) SARS-CoV and SARS-CoV-2 viruses. Multiplexed rapid antigen tests based on multicolored nanoparticles and cross-reactive antibodies and can be developed prospectively at low cost to improve preparedness for epidemic outbreaks.
MIT Department
Massachusetts Institute of Technology. Department of Biological Engineering
Terms of Use
Creative Commons Attribution-NonCommercial-NoDerivs License
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1016/j.nantod.2022.101669
