Identification of transcriptional regulators in the mouse immune system
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Regev_Identification of transcriptional.pdf
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Author(s)
Regev, Aviv
Date Issued
April 2013
Journal
Nature Immunology
Publisher
Nature Publishing Group
Citation
Jojic, Vladimir, Tal Shay, Katelyn Sylvia, Or Zuk, Xin Sun, Joonsoo Kang, Aviv Regev, et al. “Identification of transcriptional regulators in the mouse immune system.” Nature Immunology 14, no. 6 (April 28, 2013): 633-643.
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Author's final manuscript
Abstract
The differentiation of hematopoietic stem cells into cells of the immune system has been studied extensively in mammals, but the transcriptional circuitry that controls it is still only partially understood. Here, the Immunological Genome Project gene-expression profiles across mouse immune lineages allowed us to systematically analyze these circuits. To analyze this data set we developed Ontogenet, an algorithm for reconstructing lineage-specific regulation from gene-expression profiles across lineages. Using Ontogenet, we found differentiation stage–specific regulators of mouse hematopoiesis and identified many known hematopoietic regulators and 175 previously unknown candidate regulators, as well as their target genes and the cell types in which they act. Among the previously unknown regulators, we emphasize the role of ETV5 in the differentiation of γδ T cells. As the transcriptional programs of human and mouse cells are highly conserved, it is likely that many lessons learned from the mouse model apply to humans.
MIT Department
Massachusetts Institute of Technology. Department of Biology
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Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
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DOI of Published Version
https://doi.org/10.1038/ni.2587
