Probing Small-Molecule Microarrays with Tagged Proteins in Cell Lysates
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Author(s)
Pop, Marius S.
Wassaf, Dina
Koehler, Angela Nicole
Date Issued
December 2014
Journal
Current Protocols in Chemical Biology
Publisher
Wiley Blackwell
Citation
Pop, Marius S., Dina Wassaf, and Angela N. Koehler. “Probing Small-Molecule Microarrays with Tagged Proteins in Cell Lysates.” Current Protocols in Chemical Biology (October 15, 2009): 209–220.
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Author's final manuscript
Abstract
The technique of small-molecule microarray (SMM) screening is based on the ability of small molecules to bind to various soluble proteins. This type of interaction is easily detected by the presence of a fluorescence signal produced by labeled antibodies that specifically recognize a unique sequence (tag) present on the target protein. The fluorescent signal intensity values are determined based on signal-to-noise ratios (SNRs). SMM screening is a high-throughput, unbiased method that can rapidly identify novel direct ligands for various protein targets. This binding-based assay format is generally applicable to most proteins, but it is especially useful for protein targets that do not possess an enzymatic activity. SMMs enable screening a protein in a purified form or in the context of a cellular lysate, likely providing a more physiologically relevant screening environment.
MIT Department
Massachusetts Institute of Technology. Department of Biological Engineering
Koch Institute for Integrative Cancer Research at MIT
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DOI of Published Version
https://doi.org/10.1002/9780470559277.ch140101
