Impact of Dissociation Constant on the Detection Sensitivity of Polymerization-Based Signal Amplification Reactions
Name
Kaastrup Chan Sikes 2013.pdf
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2.76 MB
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Author(s)
Kaastrup, Kaja
Chan, Leslie
Sikes, Hadley
Date Issued
August 2013
Journal
Analytical Chemistry
Publisher
American Chemical Society (ACS)
Citation
Kaastrup, Kaja, Leslie Chan, and Hadley D. Sikes. “Impact of Dissociation Constant on the Detection Sensitivity of Polymerization-Based Signal Amplification Reactions.” Analytical Chemistry 85, no. 17 (September 3, 2013): 8055–8060.
Version
Author's final manuscript
Abstract
Many studies have demonstrated the concept of using free-radical polymerization reactions to provide signal amplification so that molecular recognition events indicative of disease states may be detected in a simple and low-cost manner. We provide the first systematic study of how the dissociation constant impacts detection sensitivity in these assays, having chosen a range of dissociation constants (nanomolar to picomolar) that is typical of those encountered in molecular diagnostic applications that detect protein–protein binding events. In addition, we use experimental results to validate a mass-action kinetic model that may be used to predict assay performance as an alternative or supplement to the empirical approach to developing new polymerization-based amplification assays that has characterized the field to date.
MIT Department
Massachusetts Institute of Technology. Department of Chemical Engineering
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DOI of Published Version
https://doi.org/10.1021/ac4018988
