Polymer multilayer tattooing for enhanced DNA vaccination
Name
Hammond_Polymer multilayer.pdf
Size
3.62 MB
Format
Adobe PDF
Checksum (MD5)
b545d14c409690e2b62ee0c44081ac06
Author(s)
DeMuth, Peter Charles
Min, Younjin
Huang, Bonnie
Kramer, Joshua A.
Miller, Andrew D.
Barouch, Dan H.
Hammond, Paula T.
Irvine, Darrell J.
Date Issued
January 2013
Journal
Nature Materials
Publisher
Nature Publishing Group
Citation
DeMuth, Peter C., Younjin Min, Bonnie Huang, Joshua A. Kramer, Andrew D. Miller, Dan H. Barouch, Paula T. Hammond, and Darrell J. Irvine. “Polymer Multilayer Tattooing for Enhanced DNA Vaccination.” Nature Materials 12, no. 4 (January 27, 2013): 367–376.
Version
Author's final manuscript
Abstract
DNA vaccines have many potential benefits but have failed to generate robust immune responses in humans. Recently, methods such as in vivo electroporation have demonstrated improved performance, but an optimal strategy for safe, reproducible, and pain-free DNA vaccination remains elusive. Here we report an approach for rapid implantation of vaccine-loaded polymer films carrying DNA, immune-stimulatory RNA, and biodegradable polycations into the immune-cell-rich epidermis, using microneedles coated with releasable polyelectrolyte multilayers. Films transferred into the skin following brief microneedle application promoted local transfection and controlled the persistence of DNA and adjuvants in the skin from days to weeks, with kinetics determined by the film composition. These ‘multilayer tattoo’ DNA vaccines induced immune responses against a model HIV antigen comparable to electroporation in mice, enhanced memory T-cell generation, and elicited 140-fold higher gene expression in non-human primate skin than intradermal DNA injection, indicating the potential of this strategy for enhancing DNA vaccination.
MIT Department
Massachusetts Institute of Technology. Institute for Soldier Nanotechnologies
Massachusetts Institute of Technology. Department of Biological Engineering
Massachusetts Institute of Technology. Department of Chemical Engineering
Massachusetts Institute of Technology. Department of Materials Science and Engineering
Ragon Institute of MGH, MIT and Harvard
Koch Institute for Integrative Cancer Research at MIT
Terms of Use
Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1038/nmat3550
