Integrated single-cell analysis shows Pichia pastoris secretes protein stochastically.
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LoveK Pichia_Switch_OA.pdf
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Author(s)
Love, Kerry Routenberg
Panagiotou, Vasiliki
Jiang, Bo
Stadheim, Terrance A.
Love, J. Christopher
Date Issued
June 2010
Journal
Biotechnology and Bioengineering
Publisher
John Wiley & Sons, Inc.
Citation
Love, Kerry Routenberg et al. “Integrated Single-cell Analysis Shows Pichia Pastoris Secretes Protein Stochastically.” Biotechnology and Bioengineering, 106.2 (2010): 319–325.
Version
Author's final manuscript
Abstract
The production of heterologous proteins by secretion from cellular hosts is an important determinant for the cost of biotherapeutics. A single-cell analytical method called microengraving was used to examine the heterogeneity in secretion by the methylotrophic yeast Pichia pastoris. We show that constitutive secretion of a human Fc fragment by P. pastoris is not cell-cycle dependent, but rather fluctuates between states of high and low productivity in a stochastic manner.
MIT Department
Massachusetts Institute of Technology. Department of Chemical Engineering
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Creative Commons Attribution-Noncommercial-Share Alike 3.0
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DOI of Published Version
https://doi.org/10.1002/bit.22688
