Mediator and RNA polymerase II clusters associate in transcription-dependent condensates
Name
ChoSpille-Main_Final_DSpace.pdf
Description
Accepted version
Size
1.17 MB
Format
Adobe PDF
Checksum (MD5)
301957359cb42dc3cae7c44e1281afaf
Author(s)
Cho, Won-ki
Spille, Jan Hendrik
Hecht, Micca
Lee, Choongman
Li, Charles Han
Grube, Valentin
Cisse, Ibrahim I
Date Issued
2018
Journal
Science
Publisher
American Association for the Advancement of Science (AAAS)
Citation
Cho, Won-Ki et al. "Mediator and RNA polymerase II clusters associate in transcription-dependent condensates." Science 361, 6400 (July 2018): 412-415 © 2018 Academic Press
Version
Author's final manuscript
Abstract
Models of gene control have emerged from genetic and biochemical studies, with limited consideration of the spatial organization and dynamics of key components in living cells. We used live-cell superresolution and light-sheet imaging to study the organization and dynamics of the Mediator coactivator and RNA polymerase II (Pol II) directly. Mediator and Pol II each form small transient and large stable clusters in living embryonic stem cells. Mediator and Pol II are colocalized in the stable clusters, which associate with chromatin, have properties of phase-separated condensates, and are sensitive to transcriptional inhibitors. We suggest that large clusters of Mediator, recruited by transcription factors at large or clustered enhancer elements, interact with large Pol II clusters in transcriptional condensates in vivo.
MIT Department
Massachusetts Institute of Technology. Department of Physics
Massachusetts Institute of Technology. Department of Biology
MIT Materials Research Laboratory
Terms of Use
Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1126/science.aar4199
