Automated flow peptide synthesis
Name
1054189086-MIT.pdf
Description
Full printable version
Size
18.32 MB
Format
Adobe PDF
Checksum (MD5)
eca7a6204ee6eeaa69d7100ab28aa2a8
Author(s)
Mijalis, Alexander James
Advisor(s)
Bradley L. Pentelute.
Date Issued
2018
Publisher
Massachusetts Institute of Technology
Abstract
Though reported by Merrifield nearly sixty years ago, batch solid phase peptide synthesis remains slow at minutes to hours per residue. Here we report a fully automated, flow based approach to solid phase polypeptide synthesis with amide bond formation in seven seconds and total synthesis times of forty seconds per amino acid residue. Crude peptide purities and isolated yields were comparable to standard batch solid phase peptide synthesis. Process monitoring with absorbance spectroscopy allows for the immediate detection and rapid optimization of difficult-to-synthesize peptides. This instrument is flexible and allows for synthesis of peptide nucleic acids, glycopeptides, removal of orthogonal amine protecting groups, and click chemistry on the solid phase. At full capacity, this approach to peptide synthesis can yield tens of thousands of individual 30-mer peptides per year.
Description
Thesis: Ph. D., Massachusetts Institute of Technology, Department of Chemistry, 2018.
Cataloged from PDF version of thesis.
Includes bibliographical references.
Subjects
Chemistry.
MIT Department
Massachusetts Institute of Technology. Department of Chemistry
Terms of Use
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