Catalytic Promiscuity in the Biosynthesis of Cyclic Peptide Secondary Metabolites in Planktonic Marine Cyanobacteria
Name
Chisholm_Catalytic promiscuity.pdf
Size
2.57 MB
Format
Adobe PDF
Checksum (MD5)
cf9cbcefda54439f2e87a82dcbb04414
Author(s)
Li, Bo
Sher, Daniel
Kelly, Libusha
Shi, Yanxiang
Huang, Katherine H.
Knerr, Patrick J.
Joewono, Ike
Rusch, Doug
Chisholm, Sallie (Penny)
van der Donk, Wilfred A.
Date Issued
May 2010
Journal
Proceedings of the National Academy of Sciences of the United States of America
Publisher
National Academy of Sciences (U.S.)
Citation
Li, B. et al. “Catalytic Promiscuity in the Biosynthesis of Cyclic Peptide Secondary Metabolites in Planktonic Marine Cyanobacteria.” Proceedings of the National Academy of Sciences 107.23 (2010): 10430–10435. CrossRef. Web.
Version
Final published version
Abstract
Our understanding of secondary metabolite production in bacteria has been shaped primarily by studies of attached varieties such as symbionts, pathogens, and soil bacteria. Here we show that a strain of the single-celled, planktonic marine cyanobacterium Prochlorococcus—which conducts a sizable fraction of photosynthesis in the oceans—produces many cyclic, lanthionine-containing peptides (lantipeptides). Remarkably, in Prochlorococcus MIT9313 a single promiscuous enzyme transforms up to 29 different linear ribosomally synthesized peptides into a library of polycyclic, conformationally constrained products with highly diverse ring topologies. Genes encoding this system are found in variable abundances across the oceans—with a hot spot in a Galapagos hypersaline lagoon—suggesting they play a habitat- and/or community-specific role. The extraordinarily efficient pathway for generating structural diversity enables these cyanobacteria to produce as many secondary metabolites as model antibiotic-producing bacteria, but with much smaller genomes.
MIT Department
Massachusetts Institute of Technology. Department of Civil and Environmental Engineering
Terms of Use
Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1073/pnas.0913677107
