Aptamer photoregulation in vivo
Name
Li-2014-Aptamer photoregulat.pdf
Size
1.41 MB
Format
Adobe PDF
Checksum (MD5)
8f8a7f1a50f5d47b3b55dc0601ac1c3d
Author(s)
Li, Lele
Tong, Rong
Chu, Hunghao
Wang, Weiping
Kohane, Daniel S.
Langer, Robert S
Date Issued
December 2014
Journal
Proceedings of the National Academy of Sciences
Publisher
National Academy of Sciences (U.S.)
Citation
Li, Lele, Rong Tong, Hunghao Chu, Weiping Wang, Robert Langer, and Daniel S. Kohane. “Aptamer Photoregulation in Vivo.” Proceedings of the National Academy of Sciences 111, no. 48 (November 17, 2014): 17099–17103.
Version
Final published version
Abstract
The in vivo application of aptamers as therapeutics could be improved by enhancing target-specific accumulation while minimizing off-target uptake. We designed a light-triggered system that permits spatiotemporal regulation of aptamer activity in vitro and in vivo. Cell binding by the aptamer was prevented by hybridizing the aptamer to a photo-labile complementary oligonucleotide. Upon irradiation at the tumor site, the aptamer was liberated, leading to prolonged intratumoral retention. The relative distribution of the aptamer to the liver and kidney was also significantly decreased, compared to that of the free aptamer.
MIT Department
Massachusetts Institute of Technology. Department of Chemical Engineering
Koch Institute for Integrative Cancer Research at MIT
Terms of Use
Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1073/pnas.1420105111
