Polymeric Nanocarriers Autonomously Cross the Plant Cell Wall and Enable Protein Delivery for Stress Sensing
Name
Advanced Materials - 2024 - Zhang - Polymeric Nanocarriers Autonomously Cross the Plant Cell Wall and Enable Protein.pdf
Description
Published version
Size
7.5 MB
Format
Adobe PDF
Checksum (MD5)
1c0fb7a4111b039ce6493cab4d77edca
Author(s)
Zhang, Yilin
Cao, Yunteng
Jiang, Wenzhi
Ma, Qingquan
Shin, Jinwoo
Sun, Hui
Cui, Jianqiao
Chen, Yongsheng
Giraldo, Juan Pablo
Strano, Michael S
Date Issued
August 16, 2024
Journal
Advanced Materials
Publisher
Wiley
Citation
Zhang, Yilin, Cao, Yunteng, Jiang, Wenzhi, Ma, Qingquan, Shin, Jinwoo et al. 2024. "Polymeric Nanocarriers Autonomously Cross the Plant Cell Wall and Enable Protein Delivery for Stress Sensing." Advanced Materials, 36 (41).
Version
Final published version
Abstract
Delivery of proteins in plant cells can facilitate the design of desired functions by modulation of biological processes and plant traits but is currently limited by narrow host range, tissue damage, and poor scalability. Physical barriers in plants, including cell walls and membranes, limit protein delivery to desired plant tissues. Herein, a cationic high aspect ratio polymeric nanocarriers (PNCs) platform is developed to enable efficient protein delivery to plants. The cationic nature of PNCs binds proteins through electrostatic. The ability to precisely design PNCs’ size and aspect ratio allowed us to find a cutoff of ≈14 nm in the cell wall, below which cationic PNCs can autonomously overcome the barrier and carry their cargo into plant cells. To exploit these findings, a reduction‐oxidation sensitive green fluorescent protein (roGFP) is deployed as a stress sensor protein cargo in a model plant Nicotiana benthamiana and common crop plants, including tomato and maize. In vivo imaging of PNC‐roGFP enabled optical monitoring of plant response to wounding, biotic, and heat stressors. These results show that PNCs can be precisely designed below the size exclusion limit of cell walls to overcome current limitations in protein delivery to plants and facilitate species‐independent plant engineering.
MIT Department
Massachusetts Institute of Technology. Department of Civil and Environmental Engineering
Massachusetts Institute of Technology. Department of Chemical Engineering
Terms of Use
Creative Commons Attribution-NonCommercial-NoDerivatives
Persistent DSpace Link
DOI of Published Version
10.1002/adma.202409356
