Fast scanning two-photon microscopy
Name
698127201-MIT.pdf
Description
Full printable version
Size
4.78 MB
Format
Adobe PDF
Checksum (MD5)
d878725cf7b140877d6446ba118a599c
Author(s)
Chang, Jeremy T
Advisor(s)
Edward S. Boyden.
Alternative Title
Fast scanning 2-photon microscopy
Fast scanning two-photon microscope
Date Issued
2010
Publisher
Massachusetts Institute of Technology
Abstract
Fast scanning two-photon microscopy coupled with the use light activated ion channels provides the basis for fast imaging and stimulation in the characterization of in vivo neural networks. A two-photon microscope capable of fast scanning using acousto-optic deflectors was designed and implemented. The software controller was expanded so that random access scan in three dimensions could be handled, so that algorithms that can identify neurons from images acquired using the two-photon microscope can be developed. Finally the localization of optogenetic Channelrhodopsin-2 channel to the neuron cell body was tested using a ChR2-MBD construct.
Description
Thesis (M. Eng.)--Massachusetts Institute of Technology, Dept. of Electrical Engineering and Computer Science, 2010.
Cataloged from PDF version of thesis.
Includes bibliographical references (p. 45-46).
Subjects
Electrical Engineering and Computer Science.
MIT Department
Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science
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copyright. They may be viewed from this source for any purpose, but
reproduction or distribution in any format is prohibited without written
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