Data-Driven Prediction and Design of bZIP Coiled-Coil Interactions
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Potapov-2015-Data-driven Prediction.pdf
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Author(s)
Potapov, Vladimir
Kaplan, Jenifer B.
Keating, Amy E.
Date Issued
February 2015
Journal
PLOS Computational Biology
Publisher
Public Library of Science
Citation
Potapov, Vladimir, Jenifer B. Kaplan, and Amy E. Keating. “Data-Driven Prediction and Design of bZIP Coiled-Coil Interactions.” Edited by Robert B. Russell. PLoS Comput Biol 11, no. 2 (February 19, 2015): e1004046.
Version
Final published version
Abstract
Selective dimerization of the basic-region leucine-zipper (bZIP) transcription factors presents a vivid example of how a high degree of interaction specificity can be achieved within a family of structurally similar proteins. The coiled-coil motif that mediates homo- or hetero-dimerization of the bZIP proteins has been intensively studied, and a variety of methods have been proposed to predict these interactions from sequence data. In this work, we used a large quantitative set of 4,549 bZIP coiled-coil interactions to develop a predictive model that exploits knowledge of structurally conserved residue-residue interactions in the coiled-coil motif. Our model, which expresses interaction energies as a sum of interpretable residue-pair and triplet terms, achieves a correlation with experimental binding free energies of R = 0.68 and significantly out-performs other scoring functions. To use our model in protein design applications, we devised a strategy in which synthetic peptides are built by assembling 7-residue native-protein heptad modules into new combinations. An integer linear program was used to find the optimal combination of heptads to bind selectively to a target human bZIP coiled coil, but not to target paralogs. Using this approach, we designed peptides to interact with the bZIP domains from human JUN, XBP1, ATF4 and ATF5. Testing more than 132 candidate protein complexes using a fluorescence resonance energy transfer assay confirmed the formation of tight and selective heterodimers between the designed peptides and their targets. This approach can be used to make inhibitors of native proteins, or to develop novel peptides for applications in synthetic biology or nanotechnology.
MIT Department
Massachusetts Institute of Technology. Department of Biological Engineering
Massachusetts Institute of Technology. Department of Biology
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DOI of Published Version
https://doi.org/10.1371/journal.pcbi.1004046
