M-TUBE enables large-volume bacterial gene delivery using a high-throughput microfluidic electroporation platform
Name
journal.pbio.3001727.pdf
Description
Published version
Size
1.8 MB
Format
Adobe PDF
Checksum (MD5)
59125f729bc6d025cf45375bbd3a1954
Author(s)
Huang, Po-Hsun
Chen, Sijie
Shiver, Anthony L
Culver, Rebecca Neal
Huang, Kerwyn Casey
Buie, Cullen R
Date Issued
2022
Journal
PLoS Biology
Publisher
Public Library of Science (PLoS)
Citation
Huang, Po-Hsun, Chen, Sijie, Shiver, Anthony L, Culver, Rebecca Neal, Huang, Kerwyn Casey et al. 2022. "M-TUBE enables large-volume bacterial gene delivery using a high-throughput microfluidic electroporation platform." PLoS Biology, 20 (9).
Version
Final published version
Abstract
Conventional cuvette-based and microfluidics-based electroporation approaches for bacterial gene delivery have distinct advantages, but they are typically limited to relatively small sample volumes, reducing their utility for applications requiring high throughput such as the generation of mutant libraries. Here, we present a scalable, large-scale bacterial gene delivery approach enabled by a disposable, user-friendly microfluidic electroporation device requiring minimal device fabrication and straightforward operation. We demonstrate that the proposed device can outperform conventional cuvettes in a range of situations, including across Escherichia coli strains with a range of electroporation efficiencies, and we use its large-volume bacterial electroporation capability to generate a library of transposon mutants in the anaerobic gut commensal Bifidobacterium longum.
MIT Department
Massachusetts Institute of Technology. Department of Mechanical Engineering
Terms of Use
Creative Commons Attribution
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DOI of Published Version
https://doi.org/10.1371/JOURNAL.PBIO.3001727
