Prokaryotic innate immunity through pattern recognition of conserved viral proteins
Name
nihms-1853656.pdf
Description
Accepted version
Size
3.21 MB
Format
Adobe PDF
Checksum (MD5)
57c5d41307cdcecb081ef31182fc7182
Author(s)
Gao, Linyi Alex
Wilkinson, Max E
Strecker, Jonathan
Makarova, Kira S
Macrae, Rhiannon K
Koonin, Eugene V
Zhang, Feng
Date Issued
August 12, 2022
Journal
Science
Publisher
American Association for the Advancement of Science (AAAS)
Citation
Gao, Linyi Alex, Wilkinson, Max E, Strecker, Jonathan, Makarova, Kira S, Macrae, Rhiannon K et al. 2022. "Prokaryotic innate immunity through pattern recognition of conserved viral proteins." Science, 377 (6607).
Version
Author's final manuscript
Abstract
Many organisms have evolved specialized immune pattern-recognition receptors, including nucleotide-binding oligomerization domain–like receptors (NLRs) of the STAND superfamily that are ubiquitous in plants, animals, and fungi. Although the roles of NLRs in eukaryotic immunity are well established, it is unknown whether prokaryotes use similar defense mechanisms. Here, we show that antiviral STAND (Avs) homologs in bacteria and archaea detect hallmark viral proteins, triggering Avs tetramerization and the activation of diverse N-terminal effector domains, including DNA endonucleases, to abrogate infection. Cryo–electron microscopy reveals that Avs sensor domains recognize conserved folds, active-site residues, and enzyme ligands, allowing a single Avs receptor to detect a wide variety of viruses. These findings extend the paradigm of pattern recognition of pathogen-specific proteins across all three domains of life.
MIT Department
Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences
Terms of Use
Creative Commons Attribution-Noncommercial-Share Alike
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DOI of Published Version
https://doi.org/10.1126/science.abm4096
