Metabolic Engineering of E. coli for Enhanced Diols Production from Acetate
Name
metabolic-engineering-of-e-coli-for-enhanced-diols-production-from-acetate.pdf
Description
Published version
Size
4.79 MB
Format
Adobe PDF
Checksum (MD5)
38046f05e9ab67ae5b3a2ef1073218d2
Author(s)
Ricci, Luca
Cen, Xuecong
Zu, Yuexuan
Antonicelli, Giacomo
Chen, Zhen
Fino, Debora
Pirri, Fabrizio C
Stephanopoulos, Gregory
Woolston, Benjamin M
Re, Angela
Date Issued
April 18, 2025
Journal
ACS Synthetic Biology
Publisher
American Chemical Society
Citation
Metabolic Engineering of E. coli for Enhanced Diols Production from Acetate. Luca Ricci, Xuecong Cen, Yuexuan Zu, Giacomo Antonicelli, Zhen Chen, Debora Fino, Fabrizio C. Pirri, Gregory Stephanopoulos, Benjamin M. Woolston, and Angela Re. ACS Synthetic Biology 2025 14 (4), 1204-1219.
Version
Final published version
Abstract
Effective employment of renewable carbon sources is highly demanded to develop sustainable biobased manufacturing. Here, we developed Escherichia coli strains to produce 2,3-butanediol and acetoin (collectively referred to as diols) using acetate as the sole carbon source by stepwise metabolic engineering. When tested in fed-batch experiments, the strain overexpressing the entire acetate utilization pathway was found to consume acetate at a 15% faster rate (0.78 ± 0.05 g/g/h) and to produce a 35% higher diol titer (1.16 ± 0.01 g/L) than the baseline diols-producing strain. Moreover, singularly overexpressing the genes encoding alternative acetate uptake pathways as well as alternative isoforms of genes in the malate-to-pyruvate pathway unveiled that leveraging ackA-pta and maeA is more effective in enhancing acetate consumption and diols production, compared to acs and maeB. Finally, the increased substrate consumption rate and diol production obtained in flask-based experiments were confirmed in bench-scale bioreactors operated in fed-batch mode. Consequently, the highest titer of 1.56 g/L achieved in this configuration increased by over 30% compared to the only other similar effort carried out so far.
MIT Department
Massachusetts Institute of Technology. Department of Chemical Engineering
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DOI of Published Version
https://doi.org/10.1021/acssynbio.4c00839
