Cas9 gRNA engineering for genome editing, activation and repression
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Collins_Cas9 gRNA.pdf
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Author(s)
Chavez, Alejandro
Tuttle, Marcelle
Chari, Raj
Ter-Ovanesyan, Dmitry
Qian, Jason
Pruitt, Benjamin W
Buchthal, Joanna
Church, George
Kiani, Samira
Hall, Richard N.
Date Issued
September 2015
Journal
Nature Methods
Publisher
Nature Publishing Group
Citation
Kiani, Samira, Alejandro Chavez, Marcelle Tuttle, Richard N Hall, Raj Chari, Dmitry Ter-Ovanesyan, Jason Qian, et al. “Cas9 gRNA Engineering for Genome Editing, Activation and Repression.” Nature Methods 12, no. 11 (September 7, 2015): 1051–1054.
Version
Author's final manuscript
Abstract
We demonstrate that by altering the length of Cas9-associated guide RNA(gRNA) we were able to control Cas9 nuclease activity and simultaneously perform genome editing and transcriptional regulation with a single Cas9 protein. We exploited these principles to engineer mammalian synthetic circuits with combined transcriptional regulation and kill functions governed by a single multifunctional Cas9 protein.
MIT Department
MIT Synthetic Biology Center
Massachusetts Institute of Technology. Department of Biological Engineering
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Creative Commons Attribution-Noncommercial-Share Alike
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DOI of Published Version
https://doi.org/10.1038/nmeth.3580
