Analysis of Targeted and Combinatorial Approaches to Phage T7 Genome Generation
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MastersThesis.pdf
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Author(s)
Mallet, Alex
Date Issued
February 6, 2007
Abstract
I performed computational analyses of various approaches to generating re-
engineered versions of the genome of bacteriophage T7. I analyzed a proposed design for a
re-engineered genome by examining conservation of T7 genes across related phages, and
looking for RNA secondary structure arising from the re-engineered genome that might
contribute to unwanted regulation. In addition, I proposed two methods of generating
libraries of T7 genomes, and implemented simulations showing that the proposed methods
are theoretically feasible. I conclude with thoughts on how to further validate my proposed
approaches to genome generation, and suggest a specific high-throughput method of
characterizing rebuilt genomes.
Subjects
bacteriophage t7
refactoring
MIT Department
Massachusetts Institute of Technology. Department of Biological Engineering
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