A Size-Selective Intracellular Delivery Platform
Name
A size-selective.pdf
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863.53 KB
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Author(s)
Patel, Nehal
Mino-Kenudson, Mari
Thayer, Sarah P.
Liss, Andrew S.
Saung, May Tun
Sharei, Armon Reza
Adalsteinsson, Viktor A.
Cho, Nahyun
Ruiz, Camilo A.
Kirkpatrick, Jesse D.
Date Issued
November 2016
Journal
Small
Publisher
Wiley Blackwell
Citation
Saung, May Tun et al. “A Size-Selective Intracellular Delivery Platform.” Small 12, 42 (September 2016): 5873–5881 © 2016 WILEY-VCH Verlag
Version
Author's final manuscript
Abstract
Identifying and separating a subpopulation of cells from a heterogeneous mixture are essential elements of biological research. Current approaches require detailed knowledge of unique cell surface properties of the target cell population. A method is described that exploits size differences of cells to facilitate selective intracellular delivery using a high throughput microfluidic device. Cells traversing a constriction within this device undergo a transient disruption of the cell membrane that allows for cytoplasmic delivery of cargo. Unique constriction widths allow for optimization of delivery to cells of different sizes. For example, a 4 μm wide constriction is effective for delivery of cargo to primary human T-cells that have an average diameter of 6.7 μm. In contrast, a 6 or 7 μm wide constriction is best for large pancreatic cancer cell lines BxPc3 (10.8 μm) and PANC-1 (12.3 μm). These small differences in cell diameter are sufficient to allow for selective delivery of cargo to pancreatic cancer cells within a heterogeneous mixture containing T-cells. The application of this approach is demonstrated by selectively delivering dextran-conjugated fluorophores to circulating tumor cells in patient blood allowing for their subsequent isolation and genomic characterization.
MIT Department
Massachusetts Institute of Technology. Department of Chemical Engineering
Koch Institute for Integrative Cancer Research at MIT
Terms of Use
Creative Commons Attribution-Noncommercial-Share Alike
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DOI of Published Version
https://doi.org/10.1002/smll.201601155
