Transgenic labeling of parvalbumin-expressing neurons with tdTomato
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nihms718731.pdf
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Author(s)
Kaiser, Tobias
Ting, Jonathan
Monteiro, Patricia
Feng, Guoping
Date Issued
August 2015
Journal
Neuroscience
Publisher
Elsevier
Citation
Kaiser, T. et al. “Transgenic Labeling of Parvalbumin-Expressing Neurons with tdTomato.” Neuroscience 321 (May 2016): 236–245 © 2015 IBRO
Version
Author's final manuscript
Abstract
Parvalbumin (PVALB)-expressing fast-spiking interneurons subserve important roles in many brain regions by modulating circuit function and dysfunction of these neurons is strongly implicated in neuropsychiatric disorders including schizophrenia and autism. To facilitate the study of PVALB neuron function we need to be able to identify PVALB neurons in vivo. We have generated a bacterial artificial chromosome (BAC) transgenic mouse line expressing the red fluorophore tdTomato under the control of endogenous regulatory elements of the Pvalb gene locus (JAX # 027395). We show that the tdTomato transgene is faithfully expressed relative to endogenous PVALB expression throughout the brain. Furthermore, targeted patch clamp recordings confirm that the labeled populations in neocortex, striatum, and hippocampus are fast-spiking interneurons based on intrinsic properties. This new transgenic mouse line provides a useful tool to study PVALB neuron function in the normal brain as well as in mouse models of psychiatric disease.
MIT Department
Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences
McGovern Institute for Brain Research at MIT
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Creative Commons Attribution-NonCommercial-NoDerivs License
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DOI of Published Version
https://doi.org/10.1016/J.NEUROSCIENCE.2015.08.036
